Age-specific reference intervals for liver function tests in healthy neonates, infants, and young children in Iran

J Clin Lab Anal. 2023 Dec;37(23-24):e24995. doi: 10.1002/jcla.24995. Epub 2023 Dec 12.

Abstract

Background: The reference intervals (RIs) for liver function tests (LFTs) were determined in Iranian children for the first time.

Methods: A total of 344 healthy pediatrics aged 3 days to 30 months old were recruited. Serum levels of ALT, AST, ALP, direct bilirubin, and total bilirubin were measured. RIs were determined using CLSI Ep28-A3 guidelines.

Results: All analytes demonstrated age-specific differences except AST. ALT and ALP demonstrated significantly elevated levels in infants 0 to <5 months relative to the remainder of the age range. Direct and total bilirubin demonstrated markedly elevated levels in early life with mean of 0.28 mg/dL and 1.64 mg/dL observed for direct and total bilirubin, respectively, decreasing by ~50% in the adjacent partition.

Conclusion: These novel data will help improve the clinical interpretation of biochemical test results in young Iranian neonates and children and can be of value to clinical laboratories with similar populations.

Keywords: CLSI Ep28-A3 guidelines; biochemical markers; liver function tests; pediatric; reference intervals.

MeSH terms

  • Age Factors
  • Bilirubin*
  • Child
  • Child, Preschool
  • Humans
  • Infant
  • Infant, Newborn
  • Iran
  • Liver Function Tests
  • Reference Values

Substances

  • Bilirubin