Modified Cas9-Guided Oxford Nanopore Technology Sequencing Uncovers Single and Multiple Transgene Insertion Sites in a Zebrafish Melanoma Model

CRISPR J. 2023 Dec;6(6):489-492. doi: 10.1089/crispr.2023.0062.

Authors

Raffaella De Paolo^{1

2}, Uday Munagala³, Francesco Cucco^{1

2}, Samanta Sarti^{1

2}, Letizia Pitto², Filippo Martignano³, Silvestro G Conticello^{2

3}, Laura Poliseno^{1

2}

Affiliations

¹ Oncogenomics Unit, Core Research Laboratory, ISPRO, Pisa, Italy.
² Institute of Clinical Physiology, CNR, Pisa, Italy.
³ Molecular Mechanisms of Oncogenesis Unit, Core Research Laboratory, ISPRO, Florence, Italy.

PMID: 38108521
DOI: 10.1089/crispr.2023.0062

No abstract available

MeSH terms

Animals
CRISPR-Cas Systems / genetics
Gene Editing
Melanoma* / genetics
Nanopores*
Zebrafish / genetics