Extracellular vesicles of Weizmannia coagulans lilac-01 reduced cell death of primary microglia and increased mitochondrial content in dermal fibroblasts in vitro

Biosci Biotechnol Biochem. 2024 Feb 21;88(3):333-343. doi: 10.1093/bbb/zbad175.

Abstract

We investigated the properties of extracellular vesicles from the probiotic Weizmannia coagulans lilac-01 (Lilac-01EVs). The phospholipids in the Lilac-01EV membrane were phosphatidylglycerol and mitochondria-specific cardiolipin. We found that applying Lilac-01EVs to primary rat microglia in vitro resulted in a reduction in primary microglial cell death (P < .05). Lilac-01EVs, which contain cardiolipin and phosphatidylglycerol, may have the potential to inhibit cell death in primary microglia. The addition of Lilac-01EVs to senescent human dermal fibroblasts suggested that Lilac-01 EVs increase the mitochondrial content without affecting their membrane potential in these cells.

Keywords: Weizmannia coagulans lilac-01; anti-inflammation; cell death; extracellular vesicles; mitochondria.

MeSH terms

  • Animals
  • Bacillus coagulans*
  • Cardiolipins / metabolism
  • Cell Death
  • Extracellular Vesicles* / metabolism
  • Fibroblasts / metabolism
  • Humans
  • Microglia / metabolism
  • Mitochondria
  • Rats

Substances

  • Cardiolipins