Expression, purification, and characterization of anuran saxiphilins using thermofluor, fluorescence polarization, and isothermal titration calorimetry

Affiliations

• ¹ Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158-2330, USA.
• ² Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
• ³ Department of Chemistry, Stanford University, Stanford, CA 94305, USA. Electronic address: [email protected].
• ⁴ Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158-2330, USA; Departments of Biochemistry and Biophysics, and Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158-2330, USA; California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158-2330, USA; Kavli Institute for Fundamental Neuroscience, University of California, San Francisco, San Francisco, CA 94158-2330, USA; Molecular Biophysics and Integrated Bio-imaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: [email protected].