Generation of a human induced pluripotent stem cell line (OGIi001) from peripheral blood mononuclear cells of a healthy male donor

Hanmeng Zhang; Laurence Daheron; Rodrigo Cerna-Chavez; Emily M Place; Rachel M Huckfeldt; Eric A Pierce; Marcela Garita-Hernandez

doi:10.1016/j.scr.2023.103280

Generation of a human induced pluripotent stem cell line (OGIi001) from peripheral blood mononuclear cells of a healthy male donor

Stem Cell Res. 2024 Feb:74:103280. doi: 10.1016/j.scr.2023.103280. Epub 2023 Dec 13.

Authors

Hanmeng Zhang¹, Laurence Daheron², Rodrigo Cerna-Chavez¹, Emily M Place¹, Rachel M Huckfeldt¹, Eric A Pierce¹, Marcela Garita-Hernandez³

Affiliations

¹ Ocular Genomics Institute, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA, 02114, USA.
² Harvard Stem Cell Institute, Harvard University, Cambridge, MA, 02138, USA.
³ Ocular Genomics Institute, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA, 02114, USA. Electronic address: [email protected].

Abstract

We have successfully derived a novel human induced pluripotent stem cell (hiPSC) line using non-integrative Sendai virus. This hiPSC line was generated from a healthy male adult donor, aged 55, and subjected to thorough characterization and extensive quality control. The analysis confirmed the expression of undifferentiated stem cell markers, demonstrated the ability to differentiate into the three germ layers, and revealed the absence of any chromosomal abnormalities.

MeSH terms

Adult
Cell Differentiation
Cell Line
Cellular Reprogramming
Chromosome Aberrations
Humans
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear / metabolism
Male
Sendai virus / genetics

Abstract

MeSH terms

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