The case of the missing Ks: Base editor screen to assess cellular fitness at single lysines

Varun Gopala Krishna; Verena Gudrun Gautsch; Vincenzo D'Angiolella

doi:10.1016/j.molcel.2023.11.037

The case of the missing Ks: Base editor screen to assess cellular fitness at single lysines

Mol Cell. 2023 Dec 21;83(24):4442-4444. doi: 10.1016/j.molcel.2023.11.037.

Authors

Varun Gopala Krishna¹, Verena Gudrun Gautsch¹, Vincenzo D'Angiolella²

Affiliations

¹ MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
² MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK. Electronic address: [email protected].

PMID: 38134884
DOI: 10.1016/j.molcel.2023.11.037

Abstract

In this issue of Molecular Cell, Bao et al.¹ set out to elucidate "functional lysines" in the genome using adenine base editors. The study reveals several cases of alteration of functions that previous canonical CRISPR-Cas9 screens were unable to detect.

MeSH terms

Adenine
CRISPR-Cas Systems*
Gene Editing*

Substances

Adenine

Grants and funding

MR/X006980/1/MRC_/Medical Research Council/United Kingdom