Application of cell-free plasma next-generation sequencing technology in the diagnosis and management of pediatric meningitis

Nicole Duster; Nanda Ramchandar; Jennifer Foley; Lauge Farnaes; Nicole G Coufal

doi:10.1016/j.mimet.2023.106887

Application of cell-free plasma next-generation sequencing technology in the diagnosis and management of pediatric meningitis

J Microbiol Methods. 2024 Feb-Mar:217-218:106887. doi: 10.1016/j.mimet.2023.106887. Epub 2023 Dec 31.

Authors

Nicole Duster¹, Nanda Ramchandar², Jennifer Foley³, Lauge Farnaes¹, Nicole G Coufal⁴

Affiliations

¹ University of California San Diego, Department of Pediatrics, San Diego, CA, USA; Rady Children's Hospital, San Diego, CA, USA.
² University of California San Diego, Department of Pediatrics, San Diego, CA, USA; Rady Children's Hospital, San Diego, CA, USA; Naval Medical Center San Diego, San Diego, CA, USA.
³ Rady Children's Hospital, San Diego, CA, USA.
⁴ University of California San Diego, Department of Pediatrics, San Diego, CA, USA; Rady Children's Hospital, San Diego, CA, USA. Electronic address: [email protected].

PMID: 38169204
DOI: 10.1016/j.mimet.2023.106887

Abstract

This retrospective study evaluates the clinical utility of CFPNGS in the diagnosis and management of pediatric meningitis. CFPNGS identified a causative pathogen in 36% of 28 subjects, compared to 50% for diverse conventional testing (57% combined). CFPNGS may be considered as an adjunct to standard testing.

Keywords: CNS infection; Cell-free plasma sequencing; Meningitis; Metagenomics; NGS.

MeSH terms

Child
High-Throughput Nucleotide Sequencing
Humans
Meningitis* / diagnosis
Metagenomics
Retrospective Studies
Technology