Molecular genetic association of rs8099917 and rs1800795 polymorphisms in the progression of hepatitis Delta virus liver disease

J Venom Anim Toxins Incl Trop Dis. 2024 Jan 12:30:e20230025. doi: 10.1590/1678-9199-JVATITD-2023-0025. eCollection 2024.

Abstract

Background: The relationship between viral infections and host factors holds high hopes for identifying the role of Interferon Lambda 3 (IFNL3) and Interleukin 6 (IL-6) polymorphisms in the development of Chronic Liver Disease (CLD) in patients infected with hepatitis Delta virus (HDV) in the Western Brazilian Amazon.

Methods: Cross-sectional study conducted with a cohort of 40 chronic HDV patients, 27 with CLD and 13 without evident liver damage. Biological samples from the participants were analyzed using the polymerase chain reaction (PCR) technique, followed by sequencing by the automated Sanger method.

Results: The rs8099917 T allele, from the IFNL3 gene, showed a higher frequency in both groups; however, it was not possible to establish an association with HDV infection [OR = 1.42 (0.42 - 4.75; p = 0.556 (95% CI). For IL-6, the rs1800795 G allele was superior to rs1800795 C. Analyzing both distributions in the studied groups, any association with HDV was absent (p > 0.05).

Conclusion: The results suggest that the rs8099917 T/G (IFNL3) and rs1800795 G/C (IL-6) polymorphisms are not associated with the evolution of HDV in the studied population.

Keywords: Chronic liver disease; Hepatitis Delta Virus; Polymorphisms.

Grants and funding

This study was developed by a group of researchers from the Molecular Virology Laboratory, Oswaldo Cruz Foundation Rondônia - FIOCRUZ/RO together with the Tropical Medicine Research Center - CEPEM/RO, National Institute of Epidemiology of the Western Amazon, INCT EpiAmO, and the Rondonia Foundation to Support the Development of Scientific and Technological Actions and Research in the State of Rondonia - FAPERO (Process: VPPIS-003-FIO-20-2-75; INNOVATION IN THE AMAZON).