Isolating Primary Tumor Cells from the MMTV-PyMT Mouse Model and Their Use in Developing an Orthotopic Mouse Model of Breast Cancer

Andreja Kozak; Olga Vasiljeva; Georgy Mikhaylov

doi:10.1007/978-1-0716-3714-2_6

Isolating Primary Tumor Cells from the MMTV-PyMT Mouse Model and Their Use in Developing an Orthotopic Mouse Model of Breast Cancer

Methods Mol Biol. 2024:2773:59-65. doi: 10.1007/978-1-0716-3714-2_6.

Authors

Andreja Kozak¹, Olga Vasiljeva^{1

2

3}, Georgy Mikhaylov^{4

5}

Affiliations

¹ Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Ljubljana, Slovenia.
² The Jozef Stefan International Postgraduate School, Ljubljana, Slovenia.
³ OV: CytomX Therapeutics, Inc., South San Francisco, CA, USA.
⁴ Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Ljubljana, Slovenia. [email protected].
⁵ SwissLumix SARL, Lausanne, Switzerland. [email protected].

PMID: 38236536
DOI: 10.1007/978-1-0716-3714-2_6

Abstract

To study the effect of the immunologically unimpaired microenvironment on tumor progression as well as the efficacy of therapies requiring a functioning immune system, xenograft models are not suitable due to the use of immunodeficient mice. With orthotopic congenic transplantation of tumor cells into mammary tissue, we gain more control and reproducibility regarding tumor growth, while retaining a functioning immune response. Here, we provide a protocol for isolating primary tumor cells from the MMTV-PyMT mouse model and their use in developing an orthotopic mouse model of breast cancer.

Keywords: Breast cancer; Mammary adenocarcinoma; Mouse model; Orthotopic transplantation; Polyomavirus middle T antigen.

MeSH terms

Animals
Breast Neoplasms*
Disease Models, Animal
Female
Humans
Mice
Reproducibility of Results
Transplantation, Autologous
Transplants*
Tumor Microenvironment