Regulation of EZH2 protein stability: new mechanisms, roles in tumorigenesis, and roads to the clinic

EBioMedicine. 2024 Feb:100:104972. doi: 10.1016/j.ebiom.2024.104972. Epub 2024 Jan 19.

Abstract

The importance of EZH2 as a key methyltransferase has been well documented theoretically. Practically, the first EZH2 inhibitor Tazemetostat (EPZ6438), was approved by FDA in 2020 and is used in clinic. However, for most solid tumors it is not as effective as desired and the scope of clinical indications is limited, suggesting that targeting its enzymatic activity may not be sufficient. Recent technologies focusing on the degradation of EZH2 protein have drawn attention due to their potential robust effects. This review focuses on the molecular mechanisms that regulate EZH2 protein stability via post-translational modifications (PTMs), mainly including ubiquitination, phosphorylation, and acetylation. In addition, we discuss recent advancements of multiple proteolysis targeting chimeras (PROTACs) strategies and the latest degraders that can downregulate EZH2 protein. We aim to highlight future directions to expand the application of novel EZH2 inhibitors by targeting both EZH2 enzymatic activity and protein stability.

Keywords: EZH2; Post-translational modification; Protein stability; Targeted cancer therapy.

Publication types

  • Review

MeSH terms

  • Carcinogenesis / genetics
  • Cell Transformation, Neoplastic
  • Enhancer of Zeste Homolog 2 Protein* / metabolism
  • Enzyme Inhibitors
  • Humans
  • Neoplasms* / genetics
  • Neoplasms* / metabolism
  • Protein Stability

Substances

  • Enhancer of Zeste Homolog 2 Protein
  • Enzyme Inhibitors
  • EZH2 protein, human