Mobile CRISPRi moves through the complexity of bacterial genetics

Libera Lo Presti; Hannes Link

doi:10.1016/j.crmeth.2024.100697

Mobile CRISPRi moves through the complexity of bacterial genetics

Cell Rep Methods. 2024 Jan 22;4(1):100697. doi: 10.1016/j.crmeth.2024.100697.

Authors

Libera Lo Presti¹, Hannes Link²

Affiliations

¹ Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Auf der Morgenstelle 24, 72076 Tübingen, Germany; Cluster of Excellence "Controlling Microbes to Fight Infections", University of Tübingen, 72076 Tübingen, Germany.
² Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Auf der Morgenstelle 24, 72076 Tübingen, Germany; Cluster of Excellence "Controlling Microbes to Fight Infections", University of Tübingen, 72076 Tübingen, Germany; The M3 Research Center, Otfried-Müller-Straße 37, 72076 Tübingen, Germany. Electronic address: [email protected].

Abstract

In this issue of Cell Reports Methods, Rachwalski et al. describe a high-throughput method to screen genetic interactions in bacteria using a conjugative CRISPR interference (CRISPRi) plasmid. The method enables systematic studies of gene essentiality in diverse genomic and environmental contexts and is applicable to Escherichia coli as well as other bacteria.

MeSH terms

Chemical Phenomena
Clustered Regularly Interspaced Short Palindromic Repeats*
Escherichia coli*
Genomics