Abstract
Adaptation of coenzyme stimulation assays for the nutritional assessment of thiamine, riboflavin and pyridoxine on the Cobas Bio centrifugal analyser are described. Whole blood was collected into acid-citrate dextrose, which preserves the erythrocytes, prior to assay for several days. Washed erythrocytes stored at -70 degrees C and subsequently thawed, showed altered enzyme activities. The methods offer improved precision over existing procedures and take advantage of the high throughput capabilities of the instrumentation.
MeSH terms
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Anorexia Nervosa / complications
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Anorexia Nervosa / enzymology
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Centrifugation
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Clinical Enzyme Tests
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Coenzymes / blood*
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Erythrocytes / enzymology
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Flavin-Adenine Dinucleotide / metabolism
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Glutathione / metabolism
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Glutathione Reductase / blood
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Humans
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Mathematics
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NAD / metabolism
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Pyridoxine / blood*
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Riboflavin / blood*
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Thiamine / blood*
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Transketolase / blood
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Vitamin B Deficiency / blood
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Vitamin B Deficiency / diagnosis*
Substances
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Coenzymes
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NAD
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Flavin-Adenine Dinucleotide
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Glutathione Reductase
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Transketolase
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Glutathione
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Pyridoxine
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Riboflavin
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Thiamine