Objective: To establish a method for determination of Perchloroethylene (PCE) in blood by headspace gas chromatography-mass spectrometry (HS/GC-MS) . Methods: From Dctober to December 2021, A total of 3 mL blood samples were taken into a 10 mL headspace bottle, after heated at 60 ℃ for 30 mins, PCE in the top air was separated by VF-WAXms capillary column and detected by GC-MS. The retention time and external standard method were used for qualitative and quantitative analysis of PCE in samples, respectively. Results: There was good linear relationship in the range of 5.09-200.17 μg/L. The linear correlation coefficient was 0.9993.The detection limit was 0.21 μg/L and the lower limit of quantitation was 0.70 μg/L. The recovery rates of samples with different concentrations were 95.3%-103.8%. The intra-batch relative standard deviations (RSD) were 3.2%-4.6%, and inter-batch RSD was 4.0%-6.1%. The samples can be stored at 4 ℃ for three days and at -20 ℃ for seven days. Conclusion: This method is proved to be simple, practical and highly sensitive, which is suitable for the determination of PCE in blood.
目的： 建立顶空气相色谱-质谱法测定职业人群全血中四氯乙烯的分析方法。 方法： 于2021年10至12月，采3 ml血样于10 ml顶空瓶中，经60 ℃加热平衡30 min后，顶空气中的四氯乙烯经VF-WAX ms毛细管色谱柱分离，外标法定量。 结果： 血中四氯乙烯在5.09~200.17 μg/L内线性关系良好（r=0.999 3），方法检出限为0.21 μg/L，定量限为0.70 μg/L，不同浓度的样品加标回收率为95.3%~103.8%，批内相对标准偏差（RSD）为3.2%~4.6%，批间RSD为4.0%~6.1%。样品4 ℃可保存3 d，于-20 ℃可保存7 d。 结论： 本法简单实用，重现性好，灵敏度高，可应用于全血中PCE的生物检测。.
Keywords: Blood sample; Gas chromatography-mass spectrometry; Headspace analysis; Perchloroethylene (PCE).