Gelatin/Polyacrylamide-Based Antimicrobial and Self-Healing Hydrogel Film for Wound Healing Application

ACS Appl Bio Mater. 2024 Feb 19;7(2):879-891. doi: 10.1021/acsabm.3c00903. Epub 2024 Feb 7.

Abstract

In this study, a self-healing, adhesive, and superabsorbent film made of gelatin, poly(acrylamide), and boric acid (GelAA) was successfully synthesized using a free radical reaction mechanism. The optimized film showed a remarkable 2865 ± 42% water absorptivity and also exhibited excellent self-healing behavior. The GelAA films were further loaded with silver nanoclusters (AgNCs) and ursodeoxycholic acid (UDC) (loading efficiency = 10%) to develop UDC/Ag/GelAA films. The loading of AgNCs in UDC/Ag/GelAA films helped in exhibiting 99.99 ± 0.01% antibacterial activity against both Gram-positive and Gram-negative bacteria, making them very effective against bacterial infections. Additionally, UDC/Ag/GelAA films had 77.19 ± 0.52% porosity and showed 90% of UDC release in 30 h, which helps in improving the cell proliferation. Our research provides an easy but highly effective process for synthesizing a hydrogel film, which is an intriguing choice for wound healing applications without the use of antibiotics.

Keywords: antimicrobial; drug release; self-healing; silver nanoclusters; wound healing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins*
  • Anti-Bacterial Agents* / pharmacology
  • Anti-Bacterial Agents* / therapeutic use
  • Anti-Infective Agents*
  • Gelatin / pharmacology
  • Gram-Negative Bacteria
  • Gram-Positive Bacteria
  • Methylgalactosides*
  • Wound Healing

Substances

  • Anti-Bacterial Agents
  • Gelatin
  • hydrogel film
  • polyacrylamide
  • Anti-Infective Agents
  • Acrylic Resins
  • Methylgalactosides