CRISPR/CasΦ2-mediated gene editing in wheat and rye

Sanzeng Zhao; Xueying Han; Yachen Zhu; Yuwei Han; Huiyun Liu; Zhen Chen; Huifang Li; Dan Wang; Chaofan Tian; Yuting Yuan; Yajie Guo; Xiaomin Si; Daowen Wang; Xiang Ji

doi:10.1111/jipb.13624

CRISPR/CasΦ2-mediated gene editing in wheat and rye

J Integr Plant Biol. 2024 Apr;66(4):638-641. doi: 10.1111/jipb.13624. Epub 2024 Feb 13.

Authors

Sanzeng Zhao¹, Xueying Han¹, Yachen Zhu¹, Yuwei Han¹, Huiyun Liu¹, Zhen Chen², Huifang Li¹, Dan Wang¹, Chaofan Tian¹, Yuting Yuan¹, Yajie Guo¹, Xiaomin Si³, Daowen Wang^{1

4}, Xiang Ji¹

Affiliations

¹ State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
² Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
³ State Key Laboratory of Crop Stress Adaptation and Improvement, College of Agronomy, Henan University, Kaifeng, 475004, China.
⁴ The Shennong Laboratory, Zhengzhou, 450002, China.

PMID: 38351739
DOI: 10.1111/jipb.13624

Abstract

The compact CRISPR/CasΦ2 system provides a complementary genome engineering tool for efficient gene editing including cytosine and adenosine base editing in wheat and rye with high specificity, efficient use of the protospacer-adjacent motif TTN, and an alternative base-editing window.

MeSH terms

CRISPR-Cas Systems / genetics
Clustered Regularly Interspaced Short Palindromic Repeats
Gene Editing*
Secale / genetics
Triticum* / genetics

Abstract

MeSH terms

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