Protein deuteration via algal amino acids to circumvent proton back-exchange for 1H-detected solid-state NMR

Chem Commun (Camb). 2024 Mar 12;60(22):3083-3086. doi: 10.1039/d4cc00213j.

Abstract

With perdeuteration, solid-state NMR spectroscopy of large proteins suffers from incomplete amide-proton back-exchange. Using a 72 kDa micro-crystalline protein, we show that deuteration exclusively via deuterated amino acids, well-established in solution to suppress sidechain protonation without proton back-exchange obstacles, provides spectral resolution comparable to perdeuterated preparations at intermediate spinning frequencies.

MeSH terms

  • Amino Acids*
  • Magnetic Resonance Spectroscopy
  • Nuclear Magnetic Resonance, Biomolecular / methods
  • Proteins / chemistry
  • Protons*

Substances

  • Amino Acids
  • Protons
  • Proteins