Make It Shine: Labelling the ER for Light and Fluorescence Microscopy

Chris Hawes; Pengwei Wang; Verena Kriechbaumer

doi:10.1007/978-1-0716-3710-4_1

Make It Shine: Labelling the ER for Light and Fluorescence Microscopy

Methods Mol Biol. 2024:2772:1-14. doi: 10.1007/978-1-0716-3710-4_1.

Authors

Chris Hawes¹, Pengwei Wang², Verena Kriechbaumer³

Affiliations

¹ Endomembrane Structure and Function Research Group, Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.
² College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei Province, China.
³ Endomembrane Structure and Function Research Group, Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK. [email protected].

PMID: 38411802
DOI: 10.1007/978-1-0716-3710-4_1

Abstract

The ER is a highly dynamic network of tubules and membrane cisternae. Hence, imaging this organelle in its native and mobile state is of great importance. Here we describe methods of labelling the native plant ER using fluorescent proteins and lipid dyes as well as methods for immunolabelling on plant tissue.

Keywords: Endoplasmic reticulum; Fluorescent dyes; Fluorescent protein; Immunofluorescence; Labelling; Stable; Transient expression.

MeSH terms

Coloring Agents*
Microscopy, Fluorescence

Substances

Coloring Agents