Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination

Natasha Dzimitrowicz; Emily Breeze; Lorenzo Frigerio

doi:10.1007/978-1-0716-3710-4_6

Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination

Methods Mol Biol. 2024:2772:77-85. doi: 10.1007/978-1-0716-3710-4_6.

Authors

Natasha Dzimitrowicz¹, Emily Breeze¹, Lorenzo Frigerio²

Affiliations

¹ School of Life Sciences, University of Warwick, Coventry, UK.
² School of Life Sciences, University of Warwick, Coventry, UK. [email protected].

PMID: 38411807
DOI: 10.1007/978-1-0716-3710-4_6

Abstract

Imaging plant embryos at the cellular level over time is technically challenging, since the embryo, once its protective seed coat is removed, must be kept viable and unstressed on a microscope slide for the duration of the experiment. Here we describe a procedure and suitable apparatus for the visualization, over several days, of changes in endoplasmic reticulum (ER) morphology associated with the process of germination in Arabidopsis thaliana seeds. Moreover, we also present a user-friendly image analysis tool, which enables subtle perturbations in the ER network to be measured.

Keywords: Confocal microscopy; Embryo; Germination; Imaging chamber.

MeSH terms

Arabidopsis*
Diagnostic Imaging
Endoplasmic Reticulum
Germination*
Image Processing, Computer-Assisted
Seeds