Complete genome sequence of Methanosphaera sp. ISO3-F5, a rumen methylotrophic methanogen

Nikola Palevich; Jeyamalar Jeyanathan; Kerri Reilly; Faith P Palevich; Paul H Maclean; Dong Li; Eric Altermann; William J Kelly; Sinead C Leahy; Graeme T Attwood; Ron S Ronimus; Gemma Henderson; Peter H Janssen

doi:10.1128/mra.00043-24

Complete genome sequence of Methanosphaera sp. ISO3-F5, a rumen methylotrophic methanogen

Microbiol Resour Announc. 2024 Apr 11;13(4):e0004324. doi: 10.1128/mra.00043-24. Epub 2024 Mar 1.

Authors

Nikola Palevich¹, Jeyamalar Jeyanathan^{1

2}, Kerri Reilly¹, Faith P Palevich^{1

3}, Paul H Maclean¹, Dong Li¹, Eric Altermann^{4

5}, William J Kelly¹, Sinead C Leahy^{1

6}, Graeme T Attwood¹, Ron S Ronimus¹, Gemma Henderson^{1

7}, Peter H Janssen¹

Affiliations

¹ AgResearch Ltd., Grasslands Research Centre, Palmerston North, New Zealand.
² Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Sciences and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
³ AgResearch Ltd., Hopkirk Research Institute, Palmerston North, New Zealand.
⁴ School of Veterinary Science and Centre for Bioparticle Applications, Massey University, Palmerston North, New Zealand.
⁵ Blue Barn Life Sciences Ltd., Feilding, New Zealand.
⁶ New Zealand Agricultural Greenhouse Gas Research Centre (NZAGRC), Palmerston North, New Zealand.
⁷ Bacthera AG, Basel, Switzerland.

Abstract

Methanosphaera spp. are methylotrophic methanogenic archaea and members of the order Methanobacteriales with few cultured representatives. Methanosphaera sp. ISO3-F5 was isolated from sheep rumen contents in New Zealand. Here, we report its complete genome, consisting of a large chromosome and a megaplasmid (GenBank accession numbers CP118753 and CP118754, respectively).

Keywords: Methanosphaera; genome; methane; methanogen; methanol; ruminant.

Abstract

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