An Automated Visual Psychophysics Method to Measure Visual Function in Swine Preclinical Animal Model

Transl Vis Sci Technol. 2024 Mar 1;13(3):8. doi: 10.1167/tvst.13.3.8.

Abstract

Purpose: The aim of this study was to develop and validate a test to assess visual function in pigs using the visual psychophysics contrast sensitivity function.

Methods: We utilized a touchscreen along with a pellet reward dispenser to train three Göttingen pigs on a visual psychophysics test and determined their contrast sensitivity function. Images with different contrast resolutions were used as visual stimuli and presented against a control image in a two-choice test. Following animals' acclimatization and the first phase of training, the system was arranged such that animals could self-run multiple consecutive trials without human intervention.

Results: All animals were trained within a week and remembered the task with 1 day of reinforcement when tested 1 month after the last visual assessment. All trained animals performed well during the trial with minimal screen side bias, especially at contrast threshold above 40%.

Conclusions: Göttingen pigs are trainable for a visual psychophysics test and able to self-run the trial without human intervention.

Translational relevance: Contrast sensitivity is one of the key parameters to assess visual function in humans. The possibility of measuring the same parameters in a large animal model allows for a better translation and understanding of drug safety and efficacy in preclinical ophthalmology.

MeSH terms

  • Animals
  • Humans
  • Models, Animal
  • Ophthalmology*
  • Psychophysics
  • Swine