Cell-free synthesis of the Salmonella specific broad host range bacteriophage, felixO1

John A McFarlane; David Garenne; Vincent Noireaux; Steven D Bowden

doi:10.1016/j.mimet.2024.106920

Cell-free synthesis of the Salmonella specific broad host range bacteriophage, felixO1

J Microbiol Methods. 2024 May:220:106920. doi: 10.1016/j.mimet.2024.106920. Epub 2024 Mar 12.

Authors

John A McFarlane¹, David Garenne², Vincent Noireaux², Steven D Bowden³

Affiliations

¹ University of Minnesota, Department of Food Science and Nutrition, 1334 Eckles Avenue, Saint Paul, MN 55108, USA.
² University of Minnesota, Physics and Nanotechnology, 115 Union Street SE, Minneapolis, MN 55455, USA.
³ University of Minnesota, Department of Food Science and Nutrition, 1334 Eckles Avenue, Saint Paul, MN 55108, USA. Electronic address: [email protected].

PMID: 38485092
DOI: 10.1016/j.mimet.2024.106920

Abstract

Phage-based biocontrol of foodborne Salmonella is limited by the requisite use of Salmonella to propagate the phages. This limitation can be circumvented by producing Salmonella phages using a cell-free gene expression system (CFE) with a non-pathogenic chassis. Here, we produce the Salmonella phage felixO1 using an E. coli-based CFE system.

Keywords: Cell-free expression; Phage; Salmonella; felixO1.

MeSH terms

Bacteriophages* / genetics
Escherichia coli / genetics
Genome, Viral
Host Specificity
Salmonella / genetics
Salmonella Phages* / genetics