Oligonucleotide library screening for identification of virus-specific T-cell receptors

Eur J Immunol. 2024 Jun;54(6):e2350908. doi: 10.1002/eji.202350908. Epub 2024 Mar 19.

Abstract

We demonstrate an optimized oligonucleotide library-based approach for the identification of virus-reactive T-cell receptors using Epstein-Barr virus as an example. HEK293T served as antigen-presenting cells and were co-cultured with human T cells that were transduced with T-cell receptors in question. T-cell activation was detected by CD137 expression.

Keywords: Epstein–Barr virus; Oligonucleotide library; TCR antigen identification; Target epitope screening; T‐cell immunology.

Publication types

  • Letter

MeSH terms

  • Coculture Techniques
  • Gene Library*
  • HEK293 Cells
  • Herpesvirus 4, Human* / immunology
  • Humans
  • Lymphocyte Activation* / immunology
  • Receptors, Antigen, T-Cell* / genetics
  • Receptors, Antigen, T-Cell* / immunology
  • T-Lymphocytes / immunology
  • Tumor Necrosis Factor Receptor Superfamily, Member 9 / genetics
  • Tumor Necrosis Factor Receptor Superfamily, Member 9 / immunology
  • Tumor Necrosis Factor Receptor Superfamily, Member 9 / metabolism

Substances

  • Receptors, Antigen, T-Cell
  • Tumor Necrosis Factor Receptor Superfamily, Member 9