Bacillus cereus NJ01 induces SA- and ABA-mediated immunity against bacterial pathogens through the EDS1-WRKY18 module

Dacheng Wang; Lirong Wei; Jinbiao Ma; Yingqiao Wan; Keyi Huang; Yiqiong Sun; Huili Wen; Zhipeng Chen; Zijie Li; Dongli Yu; Haitao Cui; Jingni Wu; Yufeng Wu; Sun Tae Kim; Jing Zhao; Jane E Parker; Kenichi Tsuda; Chunhao Jiang; Yiming Wang

doi:10.1016/j.celrep.2024.113985

Bacillus cereus NJ01 induces SA- and ABA-mediated immunity against bacterial pathogens through the EDS1-WRKY18 module

Cell Rep. 2024 Apr 23;43(4):113985. doi: 10.1016/j.celrep.2024.113985. Epub 2024 Mar 21.

Authors

Dacheng Wang¹, Lirong Wei¹, Jinbiao Ma¹, Yingqiao Wan¹, Keyi Huang¹, Yiqiong Sun¹, Huili Wen², Zhipeng Chen², Zijie Li¹, Dongli Yu³, Haitao Cui⁴, Jingni Wu¹, Yufeng Wu², Sun Tae Kim⁵, Jing Zhao¹, Jane E Parker⁶, Kenichi Tsuda⁷, Chunhao Jiang⁸, Yiming Wang⁹

Affiliations

¹ Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
² State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Key Laboratory for Information Agriculture, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, China.
³ Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
⁴ Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
⁵ Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea.
⁶ Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
⁷ State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: [email protected].
⁸ Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: [email protected].
⁹ Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: [email protected].

PMID: 38517890
DOI: 10.1016/j.celrep.2024.113985

Abstract

Emerging evidence suggests a beneficial role of rhizobacteria in ameliorating plant disease resistance in an environment-friendly way. In this study, we characterize a rhizobacterium, Bacillus cereus NJ01, that enhances bacterial pathogen resistance in rice and Arabidopsis. Transcriptome analyses show that root inoculation of NJ01 induces the expression of salicylic acid (SA)- and abscisic acid (ABA)-related genes in Arabidopsis leaves. Genetic evidence showed that EDS1, PAD4, and WRKY18 are required for B. cereus NJ01-induced bacterial resistance. An EDS1-PAD4 complex interacts with WRKY18 and enhances its DNA binding activity. WRKY18 directly binds to the W box in the promoter region of the SA biosynthesis gene ICS1 and ABA biosynthesis genes NCED3 and NCED5 and contributes to the NJ01-induced bacterial resistance. Taken together, our findings indicate a role of the EDS1/PAD4-WRKY18 complex in rhizobacteria-induced disease resistance.

Keywords: CP: Plants; EDS1; PAD4; WRKY18; abscisic acid; salicylic acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid* / metabolism
Arabidopsis Proteins* / genetics
Arabidopsis Proteins* / metabolism
Arabidopsis* / genetics
Arabidopsis* / immunology
Arabidopsis* / microbiology
Bacillus cereus* / genetics
DNA-Binding Proteins* / genetics
DNA-Binding Proteins* / metabolism
Disease Resistance / genetics
Disease Resistance / immunology
Gene Expression Regulation, Plant
Oryza / genetics
Oryza / immunology
Oryza / microbiology
Plant Diseases* / immunology
Plant Diseases* / microbiology
Plant Immunity
Salicylic Acid* / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

Abscisic Acid
Arabidopsis Proteins
DNA-Binding Proteins
Salicylic Acid
Transcription Factors
EDS1 protein, Arabidopsis