CRP under 130 mg/L rules out the diagnosis of Legionella pneumophila serogroup 1 (URINELLA Study)

Eur J Clin Microbiol Infect Dis. 2024 Jun;43(6):1051-1059. doi: 10.1007/s10096-024-04814-x. Epub 2024 Mar 26.

Abstract

Introduction: In case of pneumonia, some biological findings are suggestive for Legionnaire's disease (LD) including C-reactive protein (CRP). A low level of CRP is predictive for negative Legionella Urinary-Antigen-Test (L-UAT).

Method: Observational retrospective study in Nord-Franche-Comté Hospital with external validation in Besançon University Hospital, France which included all adults with L-UAT performed during January 2018 to December 2022. The objective was to determine CRP optimal threshold to predict a L-UAT negative result.

Results: URINELLA included 5051 patients (83 with positive L-UAT). CRP optimal threshold was 131.9 mg/L, with a negative predictive value (NPV) at 100%, sensitivity at 100% and specificity at 58.0%. The AUC of the ROC-Curve was at 88.7% (95% CI, 86.3-91.1). External validation in Besançon Hospital patients showed an AUC at 89.8% (95% CI, 85.5-94.1) and NPV, sensitivity and specificity was respectively 99.9%, 97.6% and 59.1% for a CRP threshold at 131.9 mg/L; after exclusion of immunosuppressed patients, index sensitivity and NPV reached also 100%.

Conclusion: In case of pneumonia suspicion with a CRP level under 130 mg/L (independently of the severity) L-UAT is useless in immunocompetent patients with a NPV at 100%. We must remain cautious in patients with symptoms onset less than 48 h before CRP dosage.

Keywords: C-reactive protein; Legionellosis; Legionnaire’s disease; Negative Predictive Value; Urinary Antigen Test.

Publication types

  • Observational Study

MeSH terms

  • Adult
  • Aged
  • C-Reactive Protein* / analysis
  • Female
  • France
  • Humans
  • Legionella pneumophila* / isolation & purification
  • Legionnaires' Disease* / diagnosis
  • Legionnaires' Disease* / microbiology
  • Male
  • Middle Aged
  • Predictive Value of Tests
  • ROC Curve
  • Retrospective Studies
  • Sensitivity and Specificity
  • Serogroup

Substances

  • C-Reactive Protein