Trichoderma-secreted anthranilic acid promotes lateral root development via auxin signaling and RBOHF-induced endodermal cell wall remodeling

Yu Chen; Yansong Fu; Yanwei Xia; Youzhi Miao; Jiahui Shao; Wei Xuan; Yunpeng Liu; Weibing Xun; Qiuyan Yan; Qirong Shen; Ruifu Zhang

doi:10.1016/j.celrep.2024.114030

Trichoderma-secreted anthranilic acid promotes lateral root development via auxin signaling and RBOHF-induced endodermal cell wall remodeling

Cell Rep. 2024 Apr 23;43(4):114030. doi: 10.1016/j.celrep.2024.114030. Epub 2024 Mar 28.

Authors

Yu Chen¹, Yansong Fu¹, Yanwei Xia¹, Youzhi Miao¹, Jiahui Shao¹, Wei Xuan², Yunpeng Liu³, Weibing Xun¹, Qiuyan Yan⁴, Qirong Shen¹, Ruifu Zhang⁵

Affiliations

¹ Key Lab of Organic-Based Fertilizers of China and Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China.
² State Key Laboratory of Crop Genetics and Germplasm Enhancement and MOA Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing 210095, China.
³ State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
⁴ Institute of Wheat Research, Shanxi Agricultural University, Linfen 041000, China.
⁵ Key Lab of Organic-Based Fertilizers of China and Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: [email protected].

PMID: 38551966
DOI: 10.1016/j.celrep.2024.114030

Abstract

Trichoderma spp. have evolved the capacity to communicate with plants by producing various secondary metabolites (SMs). Nonhormonal SMs play important roles in plant root development, while specific SMs from rhizosphere microbes and their underlying mechanisms to control plant root branching are still largely unknown. In this study, a compound, anthranilic acid (2-AA), is identified from T. guizhouense NJAU4742 to promote lateral root development. Further studies demonstrate that 2-AA positively regulates auxin signaling and transport in the canonical auxin pathway. 2-AA also partly rescues the lateral root numbers of CASP1_pro:shy2-2, which regulates endodermal cell wall remodeling via an RBOHF-induced reactive oxygen species burst. In addition, our work reports another role for microbial 2-AA in the regulation of lateral root development, which is different from its better-known role in plant indole-3-acetic acid biosynthesis. In summary, this study identifies 2-AA from T. guizhouense NJAU4742, which plays versatile roles in regulating plant root development.

Keywords: CP: Plants; ROS burst; Trichoderma spp.; anthranilic acid; auxin biosynthesis; auxin signaling; auxin transport; cell wall remodeling; endodermis; lateral root development; secondary metabolite.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / growth & development
Arabidopsis / metabolism
Cell Wall* / metabolism
Gene Expression Regulation, Plant
Indoleacetic Acids* / metabolism
Plant Roots* / growth & development
Plant Roots* / metabolism
Reactive Oxygen Species / metabolism
Signal Transduction*
Trichoderma* / growth & development
Trichoderma* / metabolism
ortho-Aminobenzoates* / metabolism

Substances

Indoleacetic Acids
anthranilic acid
ortho-Aminobenzoates
Reactive Oxygen Species