Electrochemical Detection of Total Antioxidant Capacity (TAC) in Plant Tissues from Different Origins

Methods Mol Biol. 2024:2798:1-9. doi: 10.1007/978-1-0716-3826-2_1.

Abstract

Total antioxidant capacity (TAC) is a reliable indicator of antioxidant content in animal and plant samples. The different experimental approaches available allow the determination of TAC using, as a reference, diverse compounds with recognized antioxidant capacities such as Trolox, ascorbic acid, gallic acid, or melatonin. A new portable device, named BRS (BQC redox system), is now commercially available that, through an electrochemical approach, allows the determination of TAC in a simple, fast, reproducible, and robust way. In this chapter, using this portable device, a comparative analysis of the TAC is assayed in different red, citrus, and Solanaceae fruits, several Allium species, and organs of different plant species, including Arabidopsis thaliana. The obtained results demonstrate the versatility of the BRS portable device.

Keywords: Arabidopsis; Ascorbate; Electrochemistry; Fruits; Gallic acid; Garlic; Melatonin; Pepper; Tomato; Total antioxidant capacity; Trolox.

MeSH terms

  • Animals
  • Antioxidants
  • Arabidopsis*
  • Ascorbic Acid
  • Gallic Acid
  • Melatonin*
  • Vegetables

Substances

  • Antioxidants
  • Ascorbic Acid
  • Gallic Acid
  • Melatonin