Licensing effects of inflammatory factors and TLR ligands on the regenerative capacity of adipose-derived mesenchymal stem cells

Diána Szűcs; Tamás Monostori; Vanda Miklós; Zoltán G Páhi; Szilárd Póliska; Lajos Kemény; Zoltán Veréb

doi:10.3389/fcell.2024.1367242

Licensing effects of inflammatory factors and TLR ligands on the regenerative capacity of adipose-derived mesenchymal stem cells

Front Cell Dev Biol. 2024 Mar 28:12:1367242. doi: 10.3389/fcell.2024.1367242. eCollection 2024.

Authors

Diána Szűcs^#^{1

2

3}, Tamás Monostori^#^{1

2

3}, Vanda Miklós^#⁴, Zoltán G Páhi^{5

6}, Szilárd Póliska⁷, Lajos Kemény^{1

3

8}, Zoltán Veréb^{1

3

4}

Affiliations

¹ Regenerative Medicine and Cellular Pharmacology Laboratory, Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary.
² Doctoral School of Clinical Medicine, University of Szeged, Szeged, Hungary.
³ Centre of Excellence for Interdisciplinary Research, Development and Innovation, University of Szeged, Szeged, Hungary.
⁴ Biobank, University of Szeged, Szeged, Hungary.
⁵ Genome Integrity and DNA Repair Core Group, Hungarian Centre of Excellence for Molecular Medicine (HCEMM), University of Szeged, Szeged, Hungary.
⁶ Department of Pathology, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary.
⁷ Genomic Medicine and Bioinformatics Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
⁸ Hungarian Centre of Excellence for Molecular Medicine-USz Skin Research Group, University of Szeged, Szeged, Hungary.

^# Contributed equally.

Abstract

Introduction: Adipose tissue-derived mesenchymal stem cells are promising contributors to regenerative medicine, exhibiting the ability to regenerate tissues and modulate the immune system, which is particularly beneficial for addressing chronic inflammatory ulcers and wounds. Despite their inherent capabilities, research suggests that pretreatment amplifies therapeutic effectiveness. Methods: Our experimental design exposed adipose-derived mesenchymal stem cells to six inflammatory factors for 24 h. We subsequently evaluated gene expression and proteome profile alterations and observed the wound closure rate post-treatment. Results: Specific pretreatments, such as IL-1β, notably demonstrated an accelerated wound-healing process. Analysis of gene and protein expression profiles revealed alterations in pathways associated with tissue regeneration. Discussion: This suggests that licensed cells exhibit potentially higher therapeutic efficiency than untreated cells, shedding light on optimizing regenerative strategies using adipose tissue-derived stem cells.

Keywords: adipose-derived mesenchymal stem cells; immune system; inflammation; licensing; regenerative medicine.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by the National Research, Development, and Innovation Office (NKFI PD 132570 to ZV) and GINOP_PLUSZ-2.1.1-21-2022-00043 project (co-financed by the European Union and the European Regional Development Fund) ZV was supported by the Bolyai János Postdoctoral Fellowship (BO/00190/20/5). Project no. TKP2021-EGA-28 has been implemented with support from the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021-EGA funding scheme. LK has received funding from the EU’s Horizon 2020 research and innovation program under grant agreement No. 739593. The Biobank Competence Centre of the Life Sciences Cluster of the Centre of Excellence for Interdisciplinary Research, Development, and Innovation of the University of Szeged supported the research. SP was supported by Project no. TKP2021-NKTA-34 has been implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021-NKTA funding scheme.