Visualizing the crucial roles of plasma membrane and peroxynitrite during abdominal aortic aneurysm using two-photon fluorescence imaging

Tianyu Liang; Shuling Liu; Xinyu Chen; Mingyu Tian; Chengyan Wu; Xiaofei Sun; Keli Zhong; Yang Li; Taotao Qiang; Wei Hu; Lijun Tang

doi:10.1016/j.talanta.2024.126120

Visualizing the crucial roles of plasma membrane and peroxynitrite during abdominal aortic aneurysm using two-photon fluorescence imaging

Talanta. 2024 Jul 1:274:126120. doi: 10.1016/j.talanta.2024.126120. Epub 2024 Apr 16.

Authors

Tianyu Liang¹, Shuling Liu², Xinyu Chen², Mingyu Tian², Chengyan Wu², Xiaofei Sun², Keli Zhong², Yang Li³, Taotao Qiang⁴, Wei Hu⁵, Lijun Tang⁶

Affiliations

¹ College of Chemistry and Materials Engineering, College of Food Science and Technology, Bohai University, Jinzhou, 121013, China; Key Laboratory of Auxiliary Chemistry and Technology for Chemical Industry, Ministry of Education, Shaanxi University of Science and Technology, Xi'an, 710021, China; Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, 116600, China; Shaanxi Collaborative Innovation Center of Industrial Auxiliary Chemistry and Technology, Shaanxi University of Science and Technology, Xi'an, 710021, China.
² College of Chemistry and Materials Engineering, College of Food Science and Technology, Bohai University, Jinzhou, 121013, China.
³ College of Chemistry and Materials Engineering, College of Food Science and Technology, Bohai University, Jinzhou, 121013, China. Electronic address: [email protected].
⁴ Key Laboratory of Auxiliary Chemistry and Technology for Chemical Industry, Ministry of Education, Shaanxi University of Science and Technology, Xi'an, 710021, China; Shaanxi Collaborative Innovation Center of Industrial Auxiliary Chemistry and Technology, Shaanxi University of Science and Technology, Xi'an, 710021, China. Electronic address: [email protected].
⁵ Key Laboratory of Auxiliary Chemistry and Technology for Chemical Industry, Ministry of Education, Shaanxi University of Science and Technology, Xi'an, 710021, China; Shaanxi Collaborative Innovation Center of Industrial Auxiliary Chemistry and Technology, Shaanxi University of Science and Technology, Xi'an, 710021, China. Electronic address: [email protected].
⁶ College of Chemistry and Materials Engineering, College of Food Science and Technology, Bohai University, Jinzhou, 121013, China. Electronic address: [email protected].

PMID: 38640603
DOI: 10.1016/j.talanta.2024.126120

Abstract

Peroxynitrite (ONOO^-) and cell plasma membrane (CPM) are two key factors in cell pyroptosis during the progression of abdominal aortic aneurysm (AAA). However, their combined temporal and spatial roles in initiating AAA pathogenesis remain unclear. Herein, we developed a two-photon fluorescence probe, BH-Vis, enabling real-time dynamic detection of CPM and ONOO^- changes, and revealing their interplay in AAA. BH-Vis precisely targets CPM with reduced red fluorescence intensity correlating with diminished CPM tension. Concurrently, a blue shift of the fluorescence signal of BH-Vis occurs in response to ONOO^- offering a reliable ratiometric detection mode with enhanced accuracy by minimizing external testing variables. More importantly, two photon confocal imaging with palmitic acid (PA) and ganglioside (GM1) manipulation, which modulating cell pyroptosis, showcases reliable fluorescence fluctuations. This groundbreaking application of BH-Vis in a mouse AAA model demonstrates its significant potential for accurately identifying cell pyroptosis levels during AAA development.

Keywords: Cell plasma membrane and cell pyroptosis; Fluorescent probe; Two-photon confocal imaging; peroxynitrite.

MeSH terms

Animals
Aortic Aneurysm, Abdominal* / diagnostic imaging
Aortic Aneurysm, Abdominal* / metabolism
Aortic Aneurysm, Abdominal* / pathology
Cell Membrane* / chemistry
Cell Membrane* / metabolism
Fluorescent Dyes / chemistry
Humans
Male
Mice
Mice, Inbred C57BL
Optical Imaging*
Peroxynitrous Acid* / metabolism
Photons
Pyroptosis / drug effects

Substances

Peroxynitrous Acid
Fluorescent Dyes