Effects of moderate intensity training and lithium on spatial learning and memory in a rat model: The role of SIRT3 and PGC1-α expression levels and brain-derived neurotropic factor

Exp Gerontol. 2024 Jun 15:191:112442. doi: 10.1016/j.exger.2024.112442. Epub 2024 Apr 27.

Abstract

In this study we investigated the potential synergistic effects of moderate interval training (MIT) and lithium on spatial learning and memory. Forty-two male Wistar males were classified into six groups including I: Control, II: 10 mg/kg/day IP lithium (Li10), III: MIT, IV: Li10 + MIT, V: 40 mg/kg/day IP lithium (Li40), and VI: Li40 + MIT. Then, the rats underwent Morris Water Maze (MWM) test to assess their spatial memory and learning ability. Brain-derived neurotrophic factor (BDNF) density was measured by enzyme-linked immunosorbent assay (ELISA), and the expression of PGC1 and SIRT3 were assessed via qRT-PCR. The results show that MIT improves both memory and spatial learning; but lithium alone, does not cause this. Additionally, those exposed to a combination of exercise and lithium also had improved spatial learning and memory. Finally, we observed a positive role of BDNF protein, and PGC1 gene on the effects of exercise and lithium.

Keywords: BDNF; Cognition; Exercise; Lithium; PGC1-α; SIRT3.

MeSH terms

  • Animals
  • Brain-Derived Neurotrophic Factor* / metabolism
  • Lithium / pharmacology
  • Male
  • Maze Learning / drug effects
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha* / metabolism
  • Physical Conditioning, Animal* / physiology
  • Rats
  • Rats, Wistar
  • Sirtuin 3* / genetics
  • Sirtuin 3* / metabolism
  • Sirtuins
  • Spatial Learning / drug effects
  • Spatial Memory* / drug effects

Substances

  • Bdnf protein, rat
  • Brain-Derived Neurotrophic Factor
  • Lithium
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
  • Ppargc1a protein, rat
  • SIRT3 protein, rat
  • Sirtuin 3
  • Sirtuins