Estimating SARS-CoV-2 infection probabilities with serological data and a Bayesian mixture model

Sci Rep. 2024 Apr 25;14(1):9503. doi: 10.1038/s41598-024-60060-3.

Abstract

The individual results of SARS-CoV-2 serological tests measured after the first pandemic wave of 2020 cannot be directly interpreted as a probability of having been infected. Plus, these results are usually returned as a binary or ternary variable, relying on predefined cut-offs. We propose a Bayesian mixture model to estimate individual infection probabilities, based on 81,797 continuous anti-spike IgG tests from Euroimmun collected in France after the first wave. This approach used serological results as a continuous variable, and was therefore not based on diagnostic cut-offs. Cumulative incidence, which is necessary to compute infection probabilities, was estimated according to age and administrative region. In France, we found that a "negative" or a "positive" test, as classified by the manufacturer, could correspond to a probability of infection as high as 61.8% or as low as 67.7%, respectively. "Indeterminate" tests encompassed probabilities of infection ranging from 10.8 to 96.6%. Our model estimated tailored individual probabilities of SARS-CoV-2 infection based on age, region, and serological result. It can be applied in other contexts, if estimates of cumulative incidence are available.

Keywords: Bayes’ theorem; COVID-19; Mixture model; SARS-CoV-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Viral* / blood
  • Bayes Theorem*
  • COVID-19 Serological Testing / methods
  • COVID-19* / blood
  • COVID-19* / diagnosis
  • COVID-19* / epidemiology
  • COVID-19* / immunology
  • COVID-19* / virology
  • Child
  • Child, Preschool
  • Female
  • France / epidemiology
  • Humans
  • Immunoglobulin G / blood
  • Incidence
  • Infant
  • Male
  • Middle Aged
  • Probability
  • SARS-CoV-2* / immunology
  • SARS-CoV-2* / isolation & purification
  • Young Adult

Substances

  • Antibodies, Viral
  • Immunoglobulin G