Protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections

Hadil Onallah; Ortal Yerushalmy; Ron Braunstein; Sivan Alkalay-Oren; Amit Rimon; Daniel Gelman; Shunit Coppenhagen-Glazer; Ronen Hazan; Ran Nir-Paz

doi:10.1016/j.xpro.2024.102949

Protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections

STAR Protoc. 2024 Jun 21;5(2):102949. doi: 10.1016/j.xpro.2024.102949. Epub 2024 Apr 30.

Authors

Hadil Onallah¹, Ortal Yerushalmy², Ron Braunstein², Sivan Alkalay-Oren², Amit Rimon³, Daniel Gelman³, Shunit Coppenhagen-Glazer², Ronen Hazan⁴, Ran Nir-Paz⁵

Affiliations

¹ Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112102, Israel; The Israeli Phage Therapy Center (IPTC) of Hadassah Medical Center and the Hebrew University, Jerusalem 9112102, Israel; Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center (HHUMC), Jerusalem 9112000, Israel.
² The Israeli Phage Therapy Center (IPTC) of Hadassah Medical Center and the Hebrew University, Jerusalem 9112102, Israel; Institute of Biomedical and Oral Research (IBOR), Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel.
³ The Israeli Phage Therapy Center (IPTC) of Hadassah Medical Center and the Hebrew University, Jerusalem 9112102, Israel; Institute of Biomedical and Oral Research (IBOR), Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel; Tzameret, The Military Track of Medicine, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
⁴ The Israeli Phage Therapy Center (IPTC) of Hadassah Medical Center and the Hebrew University, Jerusalem 9112102, Israel; Institute of Biomedical and Oral Research (IBOR), Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel. Electronic address: [email protected].
⁵ Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112102, Israel; The Israeli Phage Therapy Center (IPTC) of Hadassah Medical Center and the Hebrew University, Jerusalem 9112102, Israel; Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center (HHUMC), Jerusalem 9112000, Israel. Electronic address: [email protected].

Abstract

Phage therapy has re-emerged as a promising treatment for non-resolving infections. Given the lack of approved phage treatments, there is a need to establish a compassionate use pipeline. Here, we present a protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections. We describe steps for consultation and request implementation, evaluating and comparing different aspects of phage activity, and phage production. We then detail procedures for multidisciplinary meetings, ethics approvals, phage therapy, and follow-up. For complete details on the use and execution of this protocol, please refer to Onallah et al.¹^,².

Keywords: Clinical Protocol; Health Sciences; Microbiology.

MeSH terms

Bacterial Infections / therapy
Bacteriophages* / physiology
Compassionate Use Trials*
Humans
Phage Therapy* / methods