Absence of ATM leads to altered NK cell function in mice

Clin Immunol. 2024 Jun:263:110233. doi: 10.1016/j.clim.2024.110233. Epub 2024 Apr 30.

Abstract

Ataxia-telangiectasia (A-T) is a rare disorder caused by genetic defects of A-T mutated (ATM) kinase, a key regulator of stress response, and characterized by neurodegeneration, immunodeficiency, and high incidence of cancer. Here we investigated NK cells in a mouse model of A-T (Atm-/-) showing that they are strongly impaired at killing tumor cells due to a block of early signaling events. On the other hand, in Atm-/- littermates with thymic lymphoma NK cell cytotoxicity is enhanced as compared with ATM-proficient mice, possibly via tumor-produced TNF-α. Results also suggest that expansion of exhausted NKG2D+ NK cells in Atm-/- mice is driven by low-level expression of stress-inducible NKG2D ligands, whereas development of thymoma expressing the high-affinity MULT1 ligand is associated with NKG2D down-regulation on NK cells. These results expand our understanding of immunodeficiency in A-T and encourage exploring NK cell biology in A-T patients in the attempt to identify cancer predictive biomarkers and novel therapeutic targets.

Keywords: ATM kinase; Ataxia-telangiectasia; Cytotoxicity; H60; MULT1; NKG2D; Natural killer cells; RAE-1; TNF-α.

MeSH terms

  • Animals
  • Ataxia Telangiectasia / genetics
  • Ataxia Telangiectasia / immunology
  • Ataxia Telangiectasia Mutated Proteins* / genetics
  • Ataxia Telangiectasia Mutated Proteins* / metabolism
  • Cytotoxicity, Immunologic
  • Histocompatibility Antigens Class I
  • Killer Cells, Natural* / immunology
  • Membrane Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NK Cell Lectin-Like Receptor Subfamily K* / genetics
  • NK Cell Lectin-Like Receptor Subfamily K* / metabolism
  • Signal Transduction
  • Thymoma / genetics
  • Thymoma / immunology
  • Thymus Neoplasms / genetics
  • Thymus Neoplasms / immunology
  • Tumor Necrosis Factor-alpha / immunology
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Klrk1 protein, mouse
  • Atm protein, mouse
  • UL16 binding protein 1, mouse