Serum neurofilament light chain levels correlate with small fiber related parameters in patients with hereditary transthyretin amyloidosis with polyneuropathy (ATTRv-PN)

Neurol Sci. 2024 Oct;45(10):5023-5032. doi: 10.1007/s10072-024-07562-0. Epub 2024 May 3.

Abstract

Background: Recent evidence suggests that both serum neurofilament light chain (sNfL) levels and small fiber related diagnostic variables may be valuable disease biomarkers of hereditary transthyretin amyloidosis with polyneuropathy (ATTRv-PN). Our study aimed to explore the relations between sNfL and small fiber related skin biopsy and quantitative sensory testing (QST) parameters in a cohort of ATTRv-PN patients and pre-symptomatic carriers.

Methods: We retrospectively analyzed data from 13 ATTRv patients and 21 pre-symptomatic carriers who underwent sNfL dosage, skin biopsy, and QST, and analyzed correlations between sNFL, intraepidermal nerve fiber density (IENFD), and cold (CDT) and warm detection thresholds (WDT).

Results: Both sNfL and small fiber related parameters significantly differed between carriers and patients (sNfL: p < 0.0001; IENFD: p = 0.0008; CDT, WDT: < 0.0001). sNFL levels were normal in all carriers, altered in 85% of patients, negatively correlated with distal IENFD (r = -0.47, p = 0.005), and significantly correlated with CDT (r = -0.68; p < 0.0001) and WDT (r = 0.57; p < 0.0001).

Conclusions: Our study showed that sNfL reliably discriminates symptomatic ATTRv-PN patients from pre-symptomatic carriers, and found significant relations between sNfL, skin biopsy, and QST small fiber related parameters, suggesting that sNfL might be a valuable biomarker of peripheral nerve involvement in ATTRv-PN and a supportive criterion for symptomatic disease transition.

Keywords: Hereditary transthyretin amyloidosis (ATTRv); Quantitative sensory testing; Serum neurofilaments light chain; Skin biopsy; Small fiber neuropathy.

MeSH terms

  • Adult
  • Aged
  • Amyloid Neuropathies, Familial* / blood
  • Amyloid Neuropathies, Familial* / complications
  • Amyloid Neuropathies, Familial* / diagnosis
  • Biomarkers / blood
  • Female
  • Humans
  • Male
  • Middle Aged
  • Nerve Fibers / pathology
  • Neurofilament Proteins* / blood
  • Polyneuropathies / blood
  • Polyneuropathies / diagnosis
  • Retrospective Studies
  • Skin / pathology

Substances

  • Neurofilament Proteins
  • neurofilament protein L
  • Biomarkers

Supplementary concepts

  • Amyloidosis, Hereditary, Transthyretin-Related