Backbone and methyl side-chain resonance assignments of the single chain Fab fragment of trastuzumab

Donald Gagné; James M Aramini; Yves Aubin

doi:10.1007/s12104-024-10177-3

Backbone and methyl side-chain resonance assignments of the single chain Fab fragment of trastuzumab

Biomol NMR Assign. 2024 Dec;18(2):119-128. doi: 10.1007/s12104-024-10177-3. Epub 2024 May 8.

Authors

Donald Gagné¹, James M Aramini², Yves Aubin^{3

4}

Affiliations

¹ Centre for Oncology, Radiopharmaceuticals and Research, Biologics and Radiotherapeutic Drugs Directorate, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, ON, K1A 0K9, Canada.
² Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.
³ Centre for Oncology, Radiopharmaceuticals and Research, Biologics and Radiotherapeutic Drugs Directorate, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, ON, K1A 0K9, Canada. [email protected].
⁴ Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada. [email protected].

Abstract

Trastuzumab is a therapeutic monoclonal antibody developed to target human epidermal growth factor receptor 2 (HER2) present at higher levels in early cancers. Here we report the near complete resonance assignment of trastuzumab-scFab fragment backbone and the methyl groups of isoleucine, leucine and valine residues, as well as their stereo-assignments. The antibody fragment was produced using a single chain approach in Escherichia coli.

Keywords: Escherichia coli; Fragment antigen binding; Monoclonal antibody; NMR spectroscopy; Trastuzumab.

MeSH terms

Amino Acid Sequence
Humans
Immunoglobulin Fab Fragments* / chemistry
Nuclear Magnetic Resonance, Biomolecular*
Trastuzumab* / chemistry

Substances

Trastuzumab
Immunoglobulin Fab Fragments