Generation of a control induced pluripotent stem cell line (SDUCHi001-A) from a healthy male donor

Chunmiao Li; Fengjuan Zhang; Xiaoyi Wang; Mengmeng Chen

doi:10.1016/j.scr.2024.103433

Generation of a control induced pluripotent stem cell line (SDUCHi001-A) from a healthy male donor

Stem Cell Res. 2024 Jun:77:103433. doi: 10.1016/j.scr.2024.103433. Epub 2024 Apr 30.

Authors

Chunmiao Li¹, Fengjuan Zhang², Xiaoyi Wang³, Mengmeng Chen⁴

Affiliations

¹ Department of Blood Transfusion, Children's Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China.
² Weifang Municipal Center for Disease Control and Prevention, Weifang, Shandong 261061, China.
³ Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China.
⁴ Department of Clinical Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, Shandong 250022, China. Electronic address: [email protected].

PMID: 38718593
DOI: 10.1016/j.scr.2024.103433

Abstract

An induced pluripotent stem cell (iPSC) line (SDUCHi001-A) was established using peripheral blood mononuclear cells (PBMCs) from a healthy 6 years old boy. Reprogramming of the PBMCs was achieved through non-integrating delivery of OCT4, SOX2, KFL4, BCL-XL, and c-MYC. The iPSC line expressed pluripotency markers, had a normal karyotype and trilineage differentiation potential.

MeSH terms

Cell Differentiation
Cell Line
Cellular Reprogramming
Child
Humans
Induced Pluripotent Stem Cells* / cytology
Induced Pluripotent Stem Cells* / metabolism
Karyotype
Leukocytes, Mononuclear / cytology
Leukocytes, Mononuclear / metabolism
Male