Secondary analysis of Staphylococcus aureus whole genomes reveals diverse antimicrobial resistance profiles

MicroPubl Biol. 2024 Apr 28:2024:10.17912/micropub.biology.000903. doi: 10.17912/micropub.biology.000903. eCollection 2024.

Abstract

Antimicrobial resistance (AMR) in microorganisms is an ongoing threat to human health across the globe. To better characterize the AMR profiles of six strains of Staphylococcus aureus , we performed a secondary analysis that consisted of the following steps: 1) download fastq files from the Sequence Read Archive, 2) perform a de novo genome assembly from the sequencing reads, 3) annotate the assembled contigs, 4) predict the presence of antimicrobial resistance genes. We predicted the presence of 75 unique genes that conferred resistance against 22 unique antimicrobial compounds.

Grants and funding

This work was funded by Brigham Young University. The funders had no role in the experimental design or interpretation of the results.