Preparation of Biologically Active Fractions Enriched with Glucuronoxylomannan, the Main Antigen of the Cryptococcal Capsule

Luisa J Jozefowicz; Marcio L Rodrigues

doi:10.1007/978-1-0716-3722-7_24

Preparation of Biologically Active Fractions Enriched with Glucuronoxylomannan, the Main Antigen of the Cryptococcal Capsule

Methods Mol Biol. 2024:2775:367-373. doi: 10.1007/978-1-0716-3722-7_24.

Authors

Luisa J Jozefowicz¹, Marcio L Rodrigues^{2

3}

Affiliations

¹ Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
² Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil. [email protected].
³ Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. [email protected].

PMID: 38758330
DOI: 10.1007/978-1-0716-3722-7_24

Abstract

Glucuronoxylomannan (GXM) is the principal capsular component in the Cryptococcus genus. This complex polysaccharide participates in numerous events related to the physiology and pathogenesis of Cryptococcus, which highlights the importance of establishing methods for its isolation and analysis. Conventional methods for GXM isolation have been extensively discussed in the literature. In this chapter, we describe two fast methods for obtaining extracellular fractions enriched with cryptococcal GXM.

Keywords: Capsule; Cryptococcus; Glucuronoxylomannan.

MeSH terms

Antigens, Fungal / immunology
Cryptococcus neoformans
Cryptococcus*
Fungal Capsules / chemistry
Fungal Capsules / metabolism
Humans
Polysaccharides* / chemistry

Substances

glucuronoxylomannan