Prognostic role of SOX2 and STAT3 expression on circulating T lymphocytes and CD44+/CD24neg cells in the locally advanced and metastatic breast cancer

J Surg Oncol. 2024 Sep;130(4):669-680. doi: 10.1002/jso.27716. Epub 2024 Jun 2.

Abstract

Background: Breast cancer (BC) is associated with a continuous increase in incidence, with high mortality rates in several countries. CD44, STAT3, and SOX2 are related to regulating of somatic cell division, tumorigenesis, and metastasis in BC.

Methods: A cross-sectional study was carried out at the Hospital de Cancer de Pernambuco (HCP) between 2017 and 2018. Fifty-one women with locally advanced (LA) and 14 with metastatic BC were included in the study.

Results: High CD44+/CD24neg and CD44+/CD24neg/SOX2+ levels in Luminal B (LB), HER2+, and triple-negative breast cancer (TNBC) compared with controls (p < 0.05). Low CD44+/CD24negSTAT3+ levels in LB, HER2+, and TNBC compared with controls (p < 0.05). High T lymphocytes, and low STAT3 + T, and SOX2 + T levels in BC patients (p < 0.05). High SOX2 + T levels in patients with axillary lymph node-negative (N0) compared with the axillary lymph node-positives (N1 and N2 groups; p < 0.05). High SOX2 + T levels in N1 compared to N2 (p < 0.05). High T lymphocytes and low SOX2 + T levels in the LA tumor compared to metastatic disease (p = 0.0007 and p = 0.02, respectively). High CD44 + /CD24negSTAT3+, and T lymphocyte levels in TNBC patients with LA tumor compared to metastatic (p < 0.05). Low STAT3 + T levels in TBNC patients with LA tumor compared to metastatic (p = 0.0266).

Conclusion: SOX2 and STAT3 expression on circulating T lymphocytes and CD44 + /CD24neg cells in peripheral blood have prognostic roles in breast cancer. SOX2 and STAT3 expression are potential predictive biomarkers of disease progression in breast cancer regardless of tumor subtype.

Keywords: SOX2; STAT3; breast cancer.

MeSH terms

  • Adult
  • Aged
  • Biomarkers, Tumor* / blood
  • Biomarkers, Tumor* / metabolism
  • Breast Neoplasms* / metabolism
  • Breast Neoplasms* / pathology
  • CD24 Antigen* / metabolism
  • Cross-Sectional Studies
  • Female
  • Follow-Up Studies
  • Humans
  • Hyaluronan Receptors* / metabolism
  • Middle Aged
  • Prognosis
  • SOXB1 Transcription Factors* / metabolism
  • STAT3 Transcription Factor* / metabolism
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism

Substances

  • SOXB1 Transcription Factors
  • STAT3 Transcription Factor
  • Hyaluronan Receptors
  • SOX2 protein, human
  • STAT3 protein, human
  • CD24 Antigen
  • CD44 protein, human
  • Biomarkers, Tumor
  • CD24 protein, human