Associations between fasting glucose rate-of-change and the missense variant, rs373863828, in an adult Samoan cohort

PLoS One. 2024 Jun 3;19(6):e0302643. doi: 10.1371/journal.pone.0302643. eCollection 2024.

Abstract

Background: The A allele of rs373863828 in CREB3 regulatory factor is associated with high Body Mass Index, but lower odds of type 2 diabetes. These associations have been replicated elsewhere, but to date all studies have been cross-sectional. Our aims were (1) to describe the development of type 2 diabetes and change in fasting glucose between 2010 and 2018 among a longitudinal cohort of adult Samoans without type 2 diabetes or who were not using diabetes medications at baseline, and (2) to examine associations between fasting glucose rate-of-change (mmol/L per year) and the A allele of rs373863828.

Methods: We describe and test differences in fasting glucose, the development of type 2 diabetes, body mass index, age, smoking status, physical activity, urbanicity of residence, and household asset scores between 2010 and 2018 among a cohort of n = 401 adult Samoans, selected to have a ~2:2:1 ratio of GG:AG: AA rs373863828 genotypes. Multivariate linear regression was used to test whether fasting glucose rate-of-change was associated with rs373863828 genotype, and other baseline variables.

Results: By 2018, fasting glucose and BMI significantly increased among all genotype groups, and a substantial portion of the sample developed type 2 diabetes mellitus. The A allele was associated with a lower fasting glucose rate-of-change (β = -0.05 mmol/L/year per allele, p = 0.058 among women; β = -0.004 mmol/L/year per allele, p = 0.863 among men), after accounting for baseline variables. Mean fasting glucose and mean BMI increased over an eight-year period and a substantial number of individuals developed type 2 diabetes by 2018. However, fasting glucose rate-of-change, and type 2 diabetes development was lower among females with AG and AA genotypes.

Conclusions: Further research is needed to understand the effect of the A allele on fasting glucose and type 2 diabetes development. Based on our observations that other risk factors increased over time, we advocate for the continued promotion for diabetes prevention and treatment programming, and the reduction of modifiable risk factors, in this setting.

MeSH terms

  • Adult
  • Aged
  • Alleles
  • Blood Glucose* / metabolism
  • Body Mass Index
  • Cohort Studies
  • Cross-Sectional Studies
  • Diabetes Mellitus, Type 2* / genetics
  • Fasting* / blood
  • Female
  • Genotype
  • Humans
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Mutation, Missense
  • Polymorphism, Single Nucleotide
  • Samoa
  • Tumor Suppressor Proteins

Substances

  • Blood Glucose
  • CREB3 regulatory factor, human
  • Tumor Suppressor Proteins

Grants and funding

This study was supported by a National Institutes of Health National Heart Lung and Blood Institute grants R01HL093093 granted to STM, R01HL133040 granted to RLM. ACR was supported by R01HL140570 granted to STM and JPD (https://www.nhlbi.nih.gov), and the NIH Fogarty International Center's Global Health Equity Scholars program D43TW010540 (https://medicine.yale.edu/yigh/ghes) co-funded by the NIH National Institute of Diabetes and Digestive and Kidney Diseases (https://www.niddk.nih.gov). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.