Applications of Quantitative PCR (qPCR) in Studies of Virus-Host Interactions

Kim R Chiok; Kaushal Baid; Arinjay Banerjee

doi:10.1007/978-1-0716-3890-3_9

Applications of Quantitative PCR (qPCR) in Studies of Virus-Host Interactions

Methods Mol Biol. 2024:2813:125-135. doi: 10.1007/978-1-0716-3890-3_9.

Authors

Kim R Chiok¹, Kaushal Baid¹, Arinjay Banerjee^{2

3

4

5

6}

Affiliations

¹ Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.
² Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada. [email protected].
³ Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada. [email protected].
⁴ Department of Biology, University of Waterloo, Waterloo, ON, Canada. [email protected].
⁵ Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada. [email protected].
⁶ Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. [email protected].

PMID: 38888775
DOI: 10.1007/978-1-0716-3890-3_9

Abstract

Emerging viruses pose significant threats to human health and the global economy. In the past two decades, three different coronaviruses have emerged to cause worldwide public health concerns. The advent of high throughput genomic and transcriptomic technologies facilitated the study of virus-host interactions, accelerating the development of diagnostics, vaccines, and therapeutics. Here, we describe quantitative PCR (qPCR) in studies of virus-host interactions to dissect host responses and viral kinetics and how these relate to one another.

Keywords: Biosafety level 3; Cell culture; PCR; Quantitative; RNA; Viral; cDNA synthesis.

MeSH terms

Animals
Host-Pathogen Interactions / genetics
Humans
RNA, Viral / genetics
Real-Time Polymerase Chain Reaction* / methods

Substances

RNA, Viral