Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research

Kim R Chiok; Nuraina A Dahlan; Arinjay Banerjee; Neeraj Dhar

doi:10.1007/978-1-0716-3890-3_10

Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research

Methods Mol Biol. 2024:2813:137-144. doi: 10.1007/978-1-0716-3890-3_10.

Authors

Kim R Chiok¹, Nuraina A Dahlan^{1

2}, Arinjay Banerjee^{1

3

4}, Neeraj Dhar^{5

6

7

8}

Affiliations

¹ Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.
² Division of Biomedical Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK, Canada.
³ Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada.
⁴ Respiratory Research Centre, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
⁵ Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada. [email protected].
⁶ Respiratory Research Centre, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada. [email protected].
⁷ Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada. [email protected].
⁸ School of Public Health, University of Saskatchewan, Saskatoon, SK, Canada. [email protected].

PMID: 38888776
DOI: 10.1007/978-1-0716-3890-3_10

Abstract

Air-liquid interface (ALI) airway culture models serve as a powerful tool to emulate the characteristic features of the respiratory tract in vitro. These models are particularly valuable for studying emerging respiratory viral and bacterial infections. Here, we describe an optimized protocol to obtain the ALI airway culture models using normal human bronchial epithelial cells (NHBECs). The protocol outlined below enables the generation of differentiated mucociliary airway epithelial cultures by day 28 following exposure to air.

Keywords: Air-liquid interface (ALI) airway culture; Host-pathogen interaction; In vitro models.

MeSH terms

Air
Bronchi / cytology
Cell Culture Techniques* / methods
Cells, Cultured
Communicable Diseases / microbiology
Epithelial Cells* / cytology
Epithelial Cells* / microbiology
Epithelial Cells* / virology
Humans
Respiratory Mucosa / cytology
Respiratory Mucosa / microbiology
Respiratory Mucosa / virology