Association of XRCC1 p. Arg194Trp gene polymorphism with the risk of hepatocellular carcinoma in HCV Egyptian population: A pilot case-control study

Int J Immunopathol Pharmacol. 2024 Jan-Dec:38:3946320241265263. doi: 10.1177/03946320241265263.

Abstract

Background: Hepatocellular carcinoma (HCC) is the most common and fatal primary liver cancer. Genetic variants of DNA repair systems can reduce DNA repair capability and increase HCC risk. Objectives: This study aimed to examine, in Egyptian hepatitis C virus (HCV) patients, the relationship between the X-ray repair cross-complementing group 1 (XRCC1) rs1799782 single nucleotide polymorphism (SNP) and HCC susceptibility. Methods: We included 100 adult HCV-positive patients with HCC and 100 adult HCV-positive patients with liver cirrhosis as pathological controls. XRCC1 rs1799782 SNP genotyping was done in both groups using quantitative real-time PCR (qPCR). The distribution of genotypes in patients and controls was compared using several inheritance models. Results: We found that the CT genotype, when analyzed under both the co-dominant (OR (95 % CI): 2.147 (1.184-3.893), p = .012) and the over-dominant (OR (95 % CI): 2.055 (1.153-3.660), p = .015) models, as well as the combined CT and TT genotypes under the dominant model (OR (95 % CI) of 1.991 (1.133-3.497), p = .017), were associated with increased susceptibility to HCC. The frequency of the T allele was higher among HCC participants (32%) compared to those with cirrhosis (23.5%) and carrying the T allele increased the risk of HCC by 1.532 times, however, these associations did not reach statistical significance (p-values >0.05). Moreover, the variant T allele was associated with worse clinical manifestations and laboratory results among the HCC group, but AFP levels were not affected significantly. Conclusions: Egyptians with XRCC1 rs1799782 SNP may have a higher risk of HCV-related HCC. More extensive multi-center prospective investigations must confirm this association.

Keywords: Egyptian; XRCC1; hepatitis C virus; hepatocellular carcinoma; single nucleotide polymorphism.

MeSH terms

  • Adult
  • Carcinoma, Hepatocellular* / epidemiology
  • Carcinoma, Hepatocellular* / genetics
  • Carcinoma, Hepatocellular* / virology
  • Case-Control Studies
  • Egypt
  • Female
  • Genetic Predisposition to Disease*
  • Genotype
  • Hepatitis C / complications
  • Hepatitis C / genetics
  • Humans
  • Liver Neoplasms* / epidemiology
  • Liver Neoplasms* / genetics
  • Liver Neoplasms* / virology
  • Male
  • Middle Aged
  • Pilot Projects
  • Polymorphism, Single Nucleotide*
  • Risk Factors
  • X-ray Repair Cross Complementing Protein 1* / genetics

Substances

  • X-ray Repair Cross Complementing Protein 1
  • XRCC1 protein, human