Protocol for the hyperinsulinemic euglycemic clamp to measure glucose kinetics in rats

Sandra Pereira; Margaret K Hahn; Bailey Humber; Talha Chaudhry; Sally Wu; Sri Mahavir Agarwal; Nanka Dimitrova; Adria Giacca

doi:10.1016/j.xpro.2024.103143

Protocol for the hyperinsulinemic euglycemic clamp to measure glucose kinetics in rats

STAR Protoc. 2024 Sep 20;5(3):103143. doi: 10.1016/j.xpro.2024.103143. Epub 2024 Jun 19.

Authors

Sandra Pereira¹, Margaret K Hahn², Bailey Humber³, Talha Chaudhry⁴, Sally Wu³, Sri Mahavir Agarwal⁵, Nanka Dimitrova⁶, Adria Giacca⁷

Affiliations

¹ Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Department of Physiology, University of Toronto, Toronto, ON, Canada. Electronic address: [email protected].
² Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada; Department of Psychiatry, University of Toronto, Toronto, ON, Canada; Banting & Best Diabetes Centre, Toronto, ON, Canada; Department of Pharmacology, University of Toronto, Toronto, ON, Canada.
³ Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada.
⁴ Department of Physiology, University of Toronto, Toronto, ON, Canada.
⁵ Schizophrenia Division, Centre for Addiction and Mental Health, Toronto, ON, Canada; Department of Psychiatry, University of Toronto, Toronto, ON, Canada; Banting & Best Diabetes Centre, Toronto, ON, Canada.
⁶ Department of Comparative Medicine, University of Toronto, Toronto, ON, Canada.
⁷ Department of Physiology, University of Toronto, Toronto, ON, Canada; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada; Banting & Best Diabetes Centre, Toronto, ON, Canada. Electronic address: [email protected].

Abstract

In rats, cannulation of the jugular vein and the carotid artery precedes the use of the hyperinsulinemic euglycemic clamp to determine insulin sensitivity in vivo. Here, we present a vascular surgery protocol to allow the infusion of substances via the vein and the collection of blood samples from the artery on the day of the hyperinsulinemic euglycemic clamp. We describe steps for preparing for and performing catheterization surgery. We then detail procedures for clamp preparation and its use. For complete details on the use and execution of this protocol, please refer to Pereira et al.¹^,²^,³.

Keywords: Health Sciences; Metabolism; Model Organisms.

MeSH terms

Animals
Blood Glucose / analysis
Blood Glucose / metabolism
Glucose / metabolism
Glucose Clamp Technique* / methods
Insulin / metabolism
Insulin Resistance* / physiology
Kinetics
Male
Rats

Substances

Glucose
Blood Glucose
Insulin