In vitro and in vivo inhibitory effects of the Sanghuang mushroom extracts against Candida albicans

Future Microbiol. 2024;19(11):983-996. doi: 10.1080/17460913.2024.2352269. Epub 2024 Jun 21.

Abstract

Aim: To explore the antifungal potential of Sanghuang mushroom, a traditional Chinese medicine. Materials & methods: The antifungal properties and the potential mechanism of Sanghuang mushroom extracts against Candida albicans were studied in vitro and in vivo. Results: Sanghuang mushroom extracts inhibited the biofilm formation, increased the cell membrane permeability and promoted cell apoptosis of C. albicans in vitro. In a murine model of vulvovaginal candidiasis, Sanghuang mushroom extracts reduced the vaginal fungal load, improved inflammatory cell infiltration and downregulated the expression of TNF-α, IL-1β and IL-6. Untargeted metabolomic analysis suggested the presence of ten antifungal components in Sanghuang mushroom extracts. Conclusion: Sanghuang mushroom extracts showed promise as antifungal agent against candidiasis, with potential therapeutic implications.

Keywords: Candida albicans; Sanghuang mushroom; antifungal; apoptosis; biofilm.

Plain language summary

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MeSH terms

  • Agaricales / chemistry
  • Animals
  • Antifungal Agents* / isolation & purification
  • Antifungal Agents* / pharmacology
  • Apoptosis / drug effects
  • Biofilms* / drug effects
  • Candida albicans* / drug effects
  • Candidiasis, Vulvovaginal* / drug therapy
  • Candidiasis, Vulvovaginal* / microbiology
  • Cell Membrane Permeability / drug effects
  • Disease Models, Animal
  • Female
  • Humans
  • Interleukin-1beta / metabolism
  • Interleukin-6 / metabolism
  • Medicine, Chinese Traditional
  • Mice
  • Microbial Sensitivity Tests
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Antifungal Agents
  • Tumor Necrosis Factor-alpha
  • Interleukin-1beta
  • Interleukin-6