We analyzed West Nile Virus (WNV) exposure from 1,222 blood donors during 2017-2018 from an area of south-central Spain. Results revealed WNV seroprevalence of 0.08% (95% CI 0.004%-0.4%) in this population. Our findings underscore the need for continued surveillance and research to manage WNV infection in this region.
Keywords: Ciudad Real; Spain; West Nile virus; blood donors; serosurvey; vector-borne infections; virus neutralization test; viruses; zoonoses.