Interrelationship among substrate utilization, metabolic productions, and housekeeping-related gene expression levels in Mortierella alpine CBS 754.68

Hamid Reza Samadlouie; Shahrokh Gharanjik; Abdolah Vatandost; Side Maryam Ghasemi Tarvigi

doi:10.1016/j.mimet.2024.106987

Interrelationship among substrate utilization, metabolic productions, and housekeeping-related gene expression levels in Mortierella alpine CBS 754.68

J Microbiol Methods. 2024 Aug:223:106987. doi: 10.1016/j.mimet.2024.106987. Epub 2024 Jul 2.

Authors

Hamid Reza Samadlouie¹, Shahrokh Gharanjik², Abdolah Vatandost³, Side Maryam Ghasemi Tarvigi³

Affiliations

¹ Department of Food Science and Technology, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran. Electronic address: [email protected].
² Department of Plant Breeding and Biotechnology, Faculty of Agricultural Engineering, Shahrood University of Technology, Shahrood, Iran.
³ Department of Food Science and Technology, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran.

PMID: 38960329
DOI: 10.1016/j.mimet.2024.106987

Abstract

The impacts of Magnesium oxide nanoparticles (MgONPs) on the expression of 10 potential housekeeping genes of Mortierella alpine were assayed. Actin emerged as the good candidate when Mortierella alpine entered the death phase subsequent to the growth phase while Dihydropteridine reductase and 28 s were identified as suitable candidates when Mortierella alpine remained in the growth phase.

Keywords: Gene expression; Housekeeping genes; Lipid; Magnesium oxide nanoparticles; Mortierella alpine; RTqPCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation, Fungal
Genes, Essential* / genetics
Mortierella* / genetics
Mortierella* / metabolism
Nanoparticles