Unveiling the synergy: Biocompatible alginate-cellulose hydrogel loaded with silk fibroin and zinc ferrite nanoparticles for enhanced cell adhesion, and anti-biofilm activity

Reza Eivazzadeh-Keihan; Seyedeh Mehrnoush Nokandeh; Hooman Aghamirza Moghim Aliabadi; Farnaz Lalebeigi; Amir Kashtiaray; Mohammad Mahdavi; Saminalsadat Sehat; Reza Ahangari Cohan; Ali Maleki

doi:10.1016/j.ijbiomac.2024.133412

Unveiling the synergy: Biocompatible alginate-cellulose hydrogel loaded with silk fibroin and zinc ferrite nanoparticles for enhanced cell adhesion, and anti-biofilm activity

Int J Biol Macromol. 2024 Aug;275(Pt 1):133412. doi: 10.1016/j.ijbiomac.2024.133412. Epub 2024 Jul 4.

Authors

Reza Eivazzadeh-Keihan¹, Seyedeh Mehrnoush Nokandeh², Hooman Aghamirza Moghim Aliabadi³, Farnaz Lalebeigi², Amir Kashtiaray², Mohammad Mahdavi⁴, Saminalsadat Sehat², Reza Ahangari Cohan⁵, Ali Maleki⁶

Affiliations

¹ Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran; Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, Iran. Electronic address: [email protected].
² Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, Iran.
³ Protein Chemistry Laboratory, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran; Advanced Chemistry Studies Lab, Department of Chemistry, K. N. Toosi University of Technology, Tehran, Iran.
⁴ Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
⁵ Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran. Electronic address: [email protected].
⁶ Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, Iran. Electronic address: [email protected].

PMID: 38968915
DOI: 10.1016/j.ijbiomac.2024.133412

Abstract

Combining a biocompatible hydrogel scaffold with the cell-supportive properties of silk fibroin (SF) and the unique functionalities of ZnFe₂O₄ nanoparticles creates a promising platform for advanced nanobiomaterials. The research is centered on synthesizing a natural hydrogel using cellulose (Cellul) and sodium alginate (SA) combined with SF and zinc ferrite nanoparticles. A range of analytical and biological assays were conducted to determine the biological and physicochemical properties of the nanobiocomposite. The hemolysis and 2,5-diphenyl-2H-tetrazolium bromide (MTT) assays indicated that the SA-Cellul hydrogel/SF/ZnFe₂O₄ nanobiocomposite was a biocompatible against human dermal fibroblasts (Hu02) and red blood cells (RBC). In addition, aside from demonstrating outstanding anti-biofilm activity, the nanobiocomposite also promotes the Hu02 cells adhesion, showcasing the synergistic effect of incorporating SF and ZnFe₂O₄ nanoparticle. These promising results show that this nanobiocomposite has potential applications in various biomedical fields.

Keywords: Cell adhesion; Cellulose; Silk fibroin; Sodium alginate; ZnFe(2)O(4).

MeSH terms

Alginates* / chemistry
Biocompatible Materials* / chemistry
Biocompatible Materials* / pharmacology
Biofilms* / drug effects
Cell Adhesion* / drug effects
Cell Line
Cellulose* / chemistry
Cellulose* / pharmacology
Ferric Compounds* / chemistry
Ferric Compounds* / pharmacology
Fibroblasts / drug effects
Fibroins* / chemistry
Fibroins* / pharmacology
Hemolysis / drug effects
Humans
Hydrogels* / chemistry
Nanoparticles / chemistry
Zinc* / chemistry

Substances

Alginates
Fibroins
Hydrogels
Cellulose
Ferric Compounds
Biocompatible Materials
Zinc
ferrite