Evaluating the use of self-conditioning adhesive combined with dual curing resin cement as an endodontic sealer: An in vitro study

Clara Isabel Anton Y Otero; Nicolas Liaudet; Enrico di Bella; Marwa Abdelaziz; Albert Feilzer; Ivo Krejci; Laurine Marger

doi:10.1080/26415275.2023.2282523

Evaluating the use of self-conditioning adhesive combined with dual curing resin cement as an endodontic sealer: An in vitro study

Biomater Investig Dent. 2023 Nov 18;10(1):2282523. doi: 10.1080/26415275.2023.2282523. eCollection 2023.

Authors

Clara Isabel Anton Y Otero¹, Nicolas Liaudet², Enrico di Bella¹, Marwa Abdelaziz¹, Albert Feilzer³, Ivo Krejci¹, Laurine Marger¹

Affiliations

¹ Division of Cariology and Endodontology, CUMD - University Clinics of Dental Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
² Bioimaging Core Facility, University of Geneva, Geneva, Switzerland.
³ Department of Dental Material Sciences, Academic Center for Dentistry Amsterdam (ACTA), Gustav Mahlerlaan, LA Amsterdam.

Abstract

Objectives: To compare infiltration, sealing and microleakage in root dentin with a self-conditioning adhesive system combined with dual curing resin (resin-based cement) to a conventional epoxy-resin-based sealer using confocal microscopy imaging.

Methods: 26 roots were enlarged and disinfected. Dentin tubules of 24 teeth were labelled with a red fluorophore (Rhodamine B) (two samples served as controls). Root canal samples were sealed in group AH (n = 11) with a conventional sealer (AH Plus Root Canal Sealer, Dentsply DeTrey) and in group RC (n = 11) with a resin-based cement (Parabond combined with Paracore, Coltène). Roots were then sectioned horizontally and immersed in H₂O₂ to remove the Rhodamine B not fixed by the sealers. The empty dentin spaces were labeled with a green fluorophore (Fluorescein) enabling the evaluation of infiltration as well as microleakage by confocal microscopy. Two additional samples were fractured in vertical direction for observation under SEM.

Results: Group RC presented significantly more infiltration in the middle third than in the middle and apical thirds of group AH. Microleakage was significantly higher in group AH than in group RC. SEM images revealed more dentin plugs and a homogenous resin layer in group RC in contrast to group AH.

Conclusion: The resin-based cement revealed promising outcomes compared to a traditional epoxy resin based sealer.

Keywords: adhesion; cement; composite resin; confocal microscopy; dentin; endodontics; infiltration; microleakage; sealer.

Plain language summary

Infiltration and microleakage in infiltrated and sealed root dentin samples are higher in middle than apical root thirds. Root dentin infiltration and sealing with a self-conditioning adhesive system and a dual-curing resin cement revealed less microleakage than with an epoxy-resin-based sealer.