Polar Lipids Supplementation Enhances Basal Excitatory Synaptic Transmission in Primary Cortical Neuron

Mol Nutr Food Res. 2024 Aug;68(15):e2300883. doi: 10.1002/mnfr.202300883. Epub 2024 Jul 10.

Abstract

Scope: Polar lipids, such as gangliosides and phospholipids, are fundamental structural components that play critical roles in the development and maturation of neurons in the brain. Recent evidence has demonstrated that dietary intakes of polar lipids in early life are associated with improved cognitive outcomes during infancy and adolescence. However, the specific mechanisms through which these lipids impact cognition remain unclear.

Methods and results: This study examines the direct physiological impact of polar lipid supplementation, in the form of buttermilk powder, on primary cortical neuron growth and maturation. The changes are measured with postsynaptic current response recordings, immunohistochemical examination of functional synapse localization and numbers, and the biochemical quantification of receptors responsible for neuronal synaptic neurotransmission. Chronic exposure to polar lipids increases primary mouse cortical neuron basal excitatory synapse response strength attributed to enhanced dendritic complexity and an altered expression of the excitatory α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit 2 (GluR2).

Conclusion: The present finding suggests that dietary polar lipids improve human cognition through an enhancement of neuronal maturation and/or function.

Keywords: dietary polar lipids; neuronal maturation; polar lipid supplementation; polar lipids; synaptic transmission.

MeSH terms

  • Animals
  • Buttermilk
  • Cells, Cultured
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / metabolism
  • Dietary Supplements*
  • Mice
  • Mice, Inbred C57BL
  • Neurons* / drug effects
  • Neurons* / physiology
  • Receptors, AMPA / metabolism
  • Synaptic Transmission* / drug effects

Substances

  • Receptors, AMPA