Loss of YAP in Schwann cells improves HNPP pathophysiology

Glia. 2024 Nov;72(11):1974-1984. doi: 10.1002/glia.24592. Epub 2024 Jul 11.

Abstract

Rapid nerve conduction in the peripheral nervous system (PNS) is facilitated by the multilamellar myelin sheath encasing many axons of peripheral nerves. Charcot-Marie-Tooth type 1A (CMT1A), and hereditary neuropathy with liability to pressure palsy (HNPP) are common demyelinating inherited peripheral neuropathies and are caused by mutations in the peripheral myelin protein 22 (PMP22) gene. Duplication of PMP22 leads to its overexpression and causes CMT1A, while its deletion results in PMP22 under expression and causes HNPP. Here, we investigated novel targets for modulating the protein level of PMP22 in HNPP. We found that genetic attenuation of the transcriptional coactivator Yap in Schwann cells reduces p-TAZ levels, increased TAZ activity, and increases PMP22 in peripheral nerves. Based on these findings, we ablated Yap alleles in Schwann cells of the Pmp22-haploinsufficient mouse model of HNPP and identified fewer tomacula on morphological assessment and improved nerve conduction in peripheral nerves. These findings suggest YAP modulation may be a new avenue for treatment of HNPP.

Keywords: HNPP; PMP22; Schwann cell; TAZ; YAP.

MeSH terms

  • Adaptor Proteins, Signal Transducing* / genetics
  • Adaptor Proteins, Signal Transducing* / metabolism
  • Animals
  • Arthrogryposis* / genetics
  • Arthrogryposis* / metabolism
  • Arthrogryposis* / physiopathology
  • Disease Models, Animal
  • Hereditary Sensory and Motor Neuropathy* / genetics
  • Hereditary Sensory and Motor Neuropathy* / metabolism
  • Hereditary Sensory and Motor Neuropathy* / physiopathology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Myelin Proteins* / genetics
  • Myelin Proteins* / metabolism
  • Neural Conduction / genetics
  • Neural Conduction / physiology
  • Schwann Cells* / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • YAP-Signaling Proteins*

Substances

  • Myelin Proteins
  • YAP-Signaling Proteins
  • Yap1 protein, mouse
  • Adaptor Proteins, Signal Transducing
  • Pmp22 protein, mouse
  • Transcription Factors
  • Wwtr1 protein, mouse

Supplementary concepts

  • Tomaculous neuropathy